Human serum amyloid A (SAA) is a non-specific marker of inflammation, the concentration of which in blood increases in response to inflammatory stimuli such as tissue injury, infection or trauma. Similarly to C-reactive protein, SAA is a major acute phase protein in human beings. SAA can be used in diagnosis, predicting outcomes and assessing the efficacy of treatment in patients with inflammation.

New MAbs

We offer several mouse monoclonal anti-SAA antibodies that are suitable for the development of immunoassays for the quantitative detection of circulating SAA in human blood. We have now launched two new MAbs, VSA6 and VSA25 that are sold under Cat.# 4SA11. In addition, three MAbs sold under Cat.# 4VS4 are suitable for the detection of human SAA in blood.

Updated pair recommendations

For the development of a sandwich immunoassay for the measurement of SAA in human plasma samples, we recommend two MAb combinations: VSA25-VSA31 and VSA6-VSA38. Please note that the antibodies in these pair recommendations are sold under two different Cat.#: 4SA11 (MAbs VSA25 and VSA6) and 4VS4 (VSA31 and VSA38).

Avoiding adsorption of SAA onto microtiter plates

According to the literature and our own data, human SAA has a tendency to adsorb non-specifically onto polystyrene surfaces of microtiter plates. This should be taken into account when designing SAA immunoassays if there is a risk of the protein being adsorbed onto surfaces. In the new Human SAA TechNotes we give a few useful tips for optimizing the assay buffers based on our own experience.

Download Human SAA TechNotes here