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Canine parvovirus type 2 (CPV-2) causes a severe and highly contagious disease in dogs. A common manifestation of parvovirus infection is hemorrhagic enteritis. The disease can be prevented by vaccination and the CPV-2 vaccine is recommended as a core vaccine that should be given to all dogs.
We now provide a recombinant CPV-2 capsid protein VP2 which could be used
• As a calibrator in immunoassays for the detection of CPV
• As an antigen in CPV antibody titer analyses
The recombinant VP2 is expressed in a eukaryotic cell line. It consists of 584 amino acid residues and does not contain any affinity tags. The protein is able to assemble into virus-like particles (VLPs, see Figure 1).
Figure 1. Electron micrographs of CPV virus-like particles that are produced by the expression of the recombinant VP2 protein.
Figure 2. The antigen is recognized by our anti-CPV antibodies (Cat. # 3PV16). The calibration curve for the 5G7-8H7 immunoassay using recombinant CPV capsid protein VP2 as the antigen.
Figure 3. SDS-PAGE of recombinant VP2. Based on SDS-PAGE, the purity of the recombinant protein exceeds 90%.
Data Sheets:
- 8CP2 Recombinant canine parvovirus VP2, antigen
- 3PV16 Canine parvovirus, antibody
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