Accurate and up-to-date HIV diagnostics remain essential for early detection, patient management, and blood screening. Assay design must continuously evolve to ensure reliable detection across genetically diverse HIV-1 strains while maintaining high performance in modern serological and combination assay formats.

Hytest supports assay developers by providing high-quality, well-characterized HIV antigens and antibodies for diverse immunoassay platforms. As part of this commitment, we are pleased to announce the launch of a new HIV-1 antigen, Cat. # 8H11, designed to support sensitive and reliable HIV assay development.

Cat. # 8H11 is a recombinant antigen expressed in E. coli. It contains HIV-1 gp120 V3 domains from groups M and O, as well as gp41 ectodomain from group M and gp41 ectodomain fragments from group O. This design provides broader epitope coverage for HIV-1 antibody detection. The construct also contains an N-terminal Trx-tag and a C-terminal His10-tag, supporting efficient purification and stability and making it a useful option for serological assay development.

This new launch complements previously launched recombinant HIV-1 antigens:

    • Cat.# 8H12 – recombinant gp41-gp120 fusion antigen

    • Cat.# 8H13 – recombinant gp41 antigen

    • Cat.# 8H16 – recombinant gp120 V3 antigen

In Hytest’s in-house evaluation, HIV-1 antigen pairs using 8H11 together with 8H12 or 8H13 demonstrated 100% sensitivity when tested with undiluted HIV-1-positive samples. In addition, both HIV-1 antigen pairs demonstrated high specificity when evaluated using various types of interference samples and clinical specimens, with specificity of ≥99.8%. Furthermore, an in-house HIV Ab/Ag combo assay prototype incorporating 8H11 demonstrated strong sensitivity and specificity performance. More details are available in our webinar recording.

Together with the HIV-2 antigens Cat. # 8H24 and 8H25 and the anti-HIV p24 monoclonal antibodies under Cat. # 3H24, Hytest offers assay developers flexible reagent options for different assay formats and development goals.

More information:

Cat.# 8H11 Datasheet

HIV technotes